Skip To Nav

Site-specific menu

Confocal/Laser Scanning Microscopy

Key Benefits


Description of the confocal

In the Confocal/Laser Scanning Microscopy, a laser light beam is expanded to make optimal use of the optics in the objective. Through a x-y deflection mechanism this beam is turned into a scanning beam, focused to a small spot by an objective lens onto a fluorescent specimen. The mixture of reflected light and emitted fluorescent light is captured by the same objective and (after conversion into a static beam by the x-y scanner device) is focused onto a photo-detector (photomultiplier) via a dichroic mirror (beam splitter). The reflected light is deviated by the dichroic mirror while the emitted fluorescent light passes through in the direction of the photomultiplier. A confocal aperture (pinhole) is placed in front of the photo-detector, such that the fluorescent light from the specimen that is not within the focal plane where the laser beam was focused will be largely obstructed by the pinhole. In this way, out-of-focus information (both above and below the focal plane) is greatly reduced. This becomes especially important when dealing with thick specimens. The spot that is focused on the center of the pinhole is often referred to as the "confocal spot."Our Zeiss Axiovert 100M inverted confocal is equipped with 3 filter sets for simultaneous or individual viewing of up to three fluorescent channels. The scope is also equipped with a transmitted light source which can be viewed alone or in concert with fluorescence. Samples can be captured as individual "slices", Z-stacks or 3D rotating animations depending on your specific requirements.


Sample images